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JACA (Japan Air Cleaning Association)

The Abstracts of the paper carried by the Journal of JACA

vol.60-4 (2022/11/30)

Enrichment and detection techniques for bioaerosol measurements

Bioaerosol contains airborne particles originating from living organisms and has significant effects on human health such as infection diseases and asthma. Effective bioaerosol measurements enables to rapidly detect dangerous airborne microbes and to establish exposure threshold to them. Bioaerosol measurements is usually based on two processes, which are enrichment and followed-by detection (identification). This paper briefly summarizes not only conventional techniques for bioaerosol enrichment and detection (identification), but also advanced techniques based on microdevices. Furthermore, we will introduce our works for bioaerosol detection at the single particle level on microdevices and discuss perspectives of bioaerosol measurements.

Sampling methodologies for measuring airborne viruses

The interest in the presence of airborne viruses has grown significantly over the recent years. The typical method to monitor airborne viruses is to collect them into a liquid and analyze the liquid suspension to enumerate the viruses. Therefore, in order to measure the airborne viruses, it is important to develop a collection technique for sampling viruses from air into a collection liquid. For monitoring in the living space, we have selected the electrostatic collection method considering the noise, and have developed a novel wet type electrostatic sampler that can collect directly into the liquid. Then, the enrichment capacity of aerosol-to-hydrosol for the influenza virus has been evaluated using the real-time quantitative polymerase chain reaction, and it has been suggested that it could be used for airborne virus monitoring. While various collection technologies have been developed, it is necessary to understand the characteristics of each and select the optimum sampling techniques.

Monitoring air-borne microorganism in cleanroom

The importance to monitor air-borne microorganism in cleanroom has been known very well since the cleanroom management has been started.
Regarding air sampling for microorganism in cleanroom, we can find relatively concrete guideline about limit in pharmaceutical industry. It is so important for the clean room management people to know the monitoring method and key guidelines from not only ISO and Pharmacopeia and risk management. It is so important to execute air monitoring after understanding monitoring methods, including product spec, risk and benefits.

Microbial Particle Counter for control of purified water and water for injection

In the pharmaceutical industries, microorganism culture method is the mainstream method for control of purified water and water for injection (WFI), but there is growing interest in Rapid Microbiological Method (RMM). By introducing the Microbial Particle Counter (MPC) developed by our company, the several problems of the culture method can be solved. This report focuses on an overview of MPC, application examples, and prospects. By using fluorescence detection and ultraviolet irradiation, this product excels in detecting microorganisms, and helps improve the quality control and purification efficiency of purified water and WFI. In addition, the operation is simple, and various statuses such as flow rate, pressure, internal leakage, and bubble detection can be constantly monitored. In recent years, due to its many advantages, the ultrafiltration membrane method has been attracting more and more attention as an alternative to the conventional (distillation) method for the purification of WFI. In the purification process using membrane for producing WFI, membrane failure can have a significant impact. In the future, this product is expected to be used in many fields requiring such quality control and anomaly detection.

Application of environmental measurement technology against infectious diseases and case studies in real environment

The infectious disease (COVID-19) caused by the novel coronavirus (SARS-CoV-2) is still having a significant impact in many parts of the world, almost three years after it has been first reported. In this situation where concerns about unknown infectious diseases will continue to be endemic, it is necessary to determine the range of acceptable risks at each activity site while implementing countermeasures based on scientific evidence to the greatest extent possible. This paper introduces some examples of the application of the author’s environmental measurement technology and the results of investigations aimed at contributing to measures against infectious diseases.